Permaul, KugenPienaar, Fernando2008-07-162008-07-162007308421http://hdl.handle.net/10321/307Submitted in fulfilment of the requirements for the Degree of Master of Technology: Biotechnology, Durban University of Technology, Durban, South Africa, 2007.The development of transgenic plants resulted in the need to utilize the various molecular methods (e.g., ELISA, real - time PCR etc.) for the detection or analysis of the presence or absence of a specific trait in a particular plant (Bt in this study). The overall aim of this study was to optimize a half – seed extraction technique as part of a laboratory protocol for transgenic maize plants and to explore the possibility of using the following molecular techniques: horizontal isoelectric focusing, real - time PCR and ELISA, as methods for detection of the Bt trait for incorporation into the half – seed extraction protocol.81 penCornCropsPlant genetic engineeringCrop improvementBiotechnology--Dissertations, AcademicCorn--Genetic engineeringCrops--Genetic engineeringCorn--BiotechnologyThesishttps://doi.org/10.51415/10321/307