Malapermal, VesharaMbatha, Joyce NonhlanhlaGengan, Robert MoonsamyAnand, Krishnan2018-06-012018-06-012015Mamapermal, V. et al. 2015. Biosynthesis of bimetallic Au-Ag nanoparticles using Ocimum basilicum (L.) with antidiabetic and antimicrobial properties. Advanced Materials Letters. 6(12): 1050-1057.0976-3961 (print)0976-397X (online)http://hdl.handle.net/10321/3005This study was aimed at developing a simple, eco-friendly and cost effective green chemistry method for the synthesis of bimetallic Au-Ag nanoparticles using Ocimum basilicum aqueous leaf and flower extracts, respectively as the natural reducing agents. The successive reduction of chloroauric acid and silver nitrate led to the formation of Au-Ag nanoparticles within 10 min at room temperature, suggesting a higher reaction rate than chemical methods involved in the synthesis. Stable, spherical nanoparticles with well-defined dimensions of average size of 3-25 nm was confirmed by UV-Visible spectroscopy, TEM, SEM-EDX, DLS, and zeta potential, whilst, FTIR in combination with GC-MS analyzed the functional groups adhered to the surface of the nanoparticles. The colloidal suspension displayed enhanced antihyperglycemic activity at 69.97 ± 3.42% (leaf) against α-amylase (from porcine) and at 85.77 ± 5.82% (flower) against Bacillus stearothermophilus α-glucosidase than that of acarbose and their respective crude extracts. Furthermore, revealed good antibacterial activity against bacterial species Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa. Copyright © 2015 VBRI Press.12 penOcimum basilicumbimetallic (Au-Ag) NPsα-amylaseα-glucosidaseDiabetesMellitusArticle10.5185/amlett.2015.5997