Guldhe, AbhishekSingh, PoonamKumari, Sheena K.Rawat, IsmailPermaul, KugenBux, Faizal2017-02-092017-02-092016Guldhe, A. et al. 2016. Biodiesel synthesis from microalgae using immobilized Aspergillus niger whole cell lipase biocatalyst. Renewable Energy. 85: 1002-1010.0960-1481 (print)1879-0682 (online)http://hdl.handle.net/10321/2243Whole cell lipase catalysis and microalgal feedstocks make overall biodiesel synthesis greener and sustainable. In this study, a novel approach of whole cell lipase-catalyzed conversion of Scenedesmus obliquus lipids was investigated for biodiesel synthesis. Microalgal biodiesel was characterized for its fuel properties. Optimization of process parameters for immobilized Aspergillus niger whole cell lipase-catalyzed biodiesel synthesis was carried out. Highest biodiesel conversion of 53.76% was achieved from S. obliquus lipids at 35 °C, methanol to oil ratio of 5:1 and 2.5% water content based on oil weight with 6 BSPs (Biomass support particles). Step-wise methanol addition was applied to account for methanol tolerance, which improved biodiesel conversion upto 80.97% and gave 90.82 ± 1.43% yield. Immobilized A. niger lipase can be used for 2 batches without significant loss in conversion efficiency. Most of the fuel properties of biodiesel met the specifications set by international standards.9 penWhole cell lipaseMicroalgaeScenedesmus obliquusBiodieselAspergillus nigerArticlehttps://doi.org/10.1016/j.renene.2015.07.059