Stenström, Thor-AxelOkoh, Anthony I.Adegoke, Anthony Ayodeji2017-09-142017-09-142016Stenström, T. A., Okoh, A. I. and Adegoke, A. A. 2016. Antibiogram of environmental isolates of Acinetobacter calcoaceticus from Nkonkobe Municipality, South Africa. 25(8): 3059-3065.1610-2304 (online)1018-4619 (print)http://hdl.handle.net/10321/2545The antibiogram of Acinetobacter isolates from freshwater and soil samples in Alice and Fort Beaufort towns in Nkonkobe Municipality, South Africa were assessed for their extended beta-lactamase (ESBL) spectrum. Eighty-six Acinetobacter isolates were obtained from the 50 samples of soil and 50 samples of water (25 in each location) analysed. The resistance of the Acinetobacter isolates ranged between 30-100% against penicillin G, ceftriazone, nitrofurantoin, erythromycin and augmentin, while 9% showed intermediate response to minocycline, and 10% were resistant to oxytetracycline. Both Tet B and Tet 39 were detected in 66.7 % of the tetracycline resistant Acinetobacter isolates and in 44.4 % of the intermediately tetracycline resistant Acinetobacter isolates. An observation of 9.3% phenotypic expression of ESBLs was made while 3.5% were carrying the blaCTX-M-1 gene; all of which were susceptible to the fluoroquinolone. The multiple antibiotic resistance (MAR) index of > 0.2 indicates that the isolates emerged from high-risk sources, in line with conventional standards. Commensal Acinetobacter spp in the environment have proven to be one of reservoirs for antibiotic resistance genes.8 penAcinetobacterCommensal antibiogramTetBTet39ESBLsOpportunistic infectionArticlehttps://doi.org/10.51415/10321/2545