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Research Publications (Health Sciences)

Permanent URI for this collectionhttp://ir-dev.dut.ac.za/handle/10321/216

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Author: search.filters.author.Krishna, Suresh Babu NaiduSubject: search.filters.subject.a-Glucosidase

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    Enhancing antidiabetic and antimicrobial performance of Ocimum basilicum, and Ocimum sanctum (L.) using silver nanoparticles
    (Saudi Biological Society, 2015-06-28) Malapermal, Veshara; Botha, Izel; Krishna, Suresh Babu Naidu; Mbatha, Joyce Nonhlanhla
    The role of silver nanoparticles (AgNps) is an attractive proposition for advancing mod-ern diabetes therapies and applied science. Stable AgNps with a size range of 3–25 nm were synthe-sized using aqueous leaf extracts from Ocimum basilicum, Ocimum sanctum, and in combination. The concentration of the extracts facilitated the reduction of silver nitrate that led to the rapid for-mation of AgNps at room temperature, indicating a higher reaction rate as opposed to harsh chem-ical methods, and high conversion energy usually involved in the synthesis. The size, shape and elemental analysis were carried out using UV–Visible spectroscopy, transmission electron micro-scopy (TEM), scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM–EDX), dynamic light scattering (DLS), and zeta potential whilst, Fourier transform infrared (FTIR) supported by gas chromatography mass spectroscopy (GC–MS) was used to identify the type of capping agents. Inhibition of a-amylase and a-glucosidase enzymes retards the rate of car-bohydrate digestion, thereby provides an alternative and a less evasive strategy of reducing post-prandial hyperglycaemia in diabetic patients. The AgNps derived from O. sanctum and O. basilicum, respectively displayed an inhibitory effect at 89.31 ± 5.32%, and 79.74 ± 9.51%, respec-tively, against Bacillus stearothermophilus a-glucosidase enzyme model, indicating an enhanced bio-catalytic potential compared to their respective crude extracts and the control. Furthermore, the emerging rate of infections in diabetic patients validates the need for the discovery of dual diabetes therapies. As a result, the bioderived AgNps displayed antimicrobial activity against bacterial species Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Salmonella species.