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Research Publications (Health Sciences)

Permanent URI for this collectionhttp://ir-dev.dut.ac.za/handle/10321/216

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Subject: search.filters.subject.α-glucosidase

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    Biosynthesis of bimetallic Au-Ag nanoparticles using Ocimum basilicum (L.) with antidiabetic and antimicrobial properties
    (VBRI Press, 2015) Malapermal, Veshara; Mbatha, Joyce Nonhlanhla; Gengan, Robert Moonsamy; Anand, Krishnan
    This study was aimed at developing a simple, eco-friendly and cost effective green chemistry method for the synthesis of bimetallic Au-Ag nanoparticles using Ocimum basilicum aqueous leaf and flower extracts, respectively as the natural reducing agents. The successive reduction of chloroauric acid and silver nitrate led to the formation of Au-Ag nanoparticles within 10 min at room temperature, suggesting a higher reaction rate than chemical methods involved in the synthesis. Stable, spherical nanoparticles with well-defined dimensions of average size of 3-25 nm was confirmed by UV-Visible spectroscopy, TEM, SEM-EDX, DLS, and zeta potential, whilst, FTIR in combination with GC-MS analyzed the functional groups adhered to the surface of the nanoparticles. The colloidal suspension displayed enhanced antihyperglycemic activity at 69.97 ± 3.42% (leaf) against α-amylase (from porcine) and at 85.77 ± 5.82% (flower) against Bacillus stearothermophilus α-glucosidase than that of acarbose and their respective crude extracts. Furthermore, revealed good antibacterial activity against bacterial species Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa. Copyright © 2015 VBRI Press.
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    Antidiabetic potential of Brachylaena discolor
    (0189-6016, 2015) Mellem, John Jason; Baijnath, Himansu; Odhav, Bharti
    Background: The traditional African herbal medicinal system has many reports of anti-diabetic food plants with no known side effects. Such plants and their products have been widely prescribed for diabetic treatment with little known mechanistic basis of their functioning. Therefore, these natural products need to be evaluated scientifically in order to confirm antidiabetic property claims. Materials and Methods: In this study, leaves of Brachylaena discolor were evaluated for potential to inhibit α-amylase and α-glucosidase. The leaves were also screened for toxicity and free radical scavenging capacity. Results: Results from the study show that the methanolic extract gave a higher α-glucosidase inhibition potential and was able to effectively scavenge free radicals better than the aqueous extract. The toxicity, cytotoxicity and mutagenicity screen also showed that both plant extracts are safe for use. Conclusion: These results therefore indicate that B. discolor has the potential for use as a potential dietary adjunct or therapy for the treatment of diabetes.