Theses and dissertations (Applied Sciences)
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Item Menu adequacy and nutritional status of children aged 6-19 years residing in child and youth care facilities in Durban(2022) Mbambo, Thembelihle Jessica Sibusisiwe; Napier, Carin E.The primary function of Child and Youth Care Facilities (CYCFs) is to provide a safe and secure environment run by trained and capable child and youth-care workers while the parents may not be capable of taking care of their children for various reasons. For children 6 - 19 years of age, the meals should provide at least 60-100% of the daily nutritional requirements. Studies worldwide have shown that child malnutrition and lack of access to healthy foods is still one of the biggest contributors to child mortality. AIM The main objective of the study was to analyse the nutritional adequacy of the menus, the food variety and the nutritional status of 6–19 year-old children residing in child and youth care facilities in Durban. METHOD Four CYCFs were randomly selected where the sample population consisted of (n=211) children between the ages of 6-19 years. The anthropometric measurements for weight and height were collected to determine BMI-for-age and height-for-age. The researcher also collected menus and recipes for analysis using Food Finder® version 3 and also conducted plate waste studies to analyse food wastage. The sample population consisted of (n=22) Child and Youth Care Workers (CYCWs) who completed the FFQ, the sociodemographic questionnaire and the nutrition knowledge questionnaire. Trained fieldworkers assisted in the interview process and the total sample size resulted in (n=233) participants. RESULTS The anthropometric indices showed a low prevalence (9.3%; n=7) of severely stunted children and stunted (9.3%; n=7) children amongst the boys. None of the children in this study were affected by wasting; however, the study indicated that 15.3% (n=21) of the girls were overweight and 16.7% (n=23) of the girls were obese. The results reported that the children were served an average portion size of 195- 287.41g of starchy foods, and the portion sizes of vegetables were below the recommended daily requirements resulting in poor micronutrient intake. The FVS in the CYCFs indicated that the highest number of individual foods consumed was between 20-40 individual foods and this indicated a medium food variety score. The results of the socio-demographic questionnaire revealed that most of the CYCWs were mothers who were the head of their households. The majority of the CYCWs had not obtained a relevant tertiary education, 72.7% had obtained a standard 10/ matric certification and 22.7% had obtained a college/FET qualification and none of the CYCWs had received training for food preparation at the CYCFs. The nutrition knowledge questionnaires indicated that more than 50% of the CYCWs answered the questions correctly CONCLUSION Overall, the majority of the meals met 100% of the daily nutrient requirements for carbohydrates, protein and fats. The majority of the micronutrients did not meet 100% of DRIs. RECOMMENDATIONS The CYCWs needed relevant training on food preparation and food handling as well as nutrition education to improve their nutrition knowledge. More nutrition interventions should be implemented to help combat nutrition deficiencies.Item Purification, application and immunolocalization of thermostable xylanases(2014) Govender, Stephanie; Singh, Suren; Permaul, Kugen; Pillai, Santhosh Kumar KuttanMicrobial enzymes are gaining worldwide attention due to their potential industrial applications. Microorganisms producing thermostable -xylanase and their associated hemicellulases have significant application in the paper and pulp, food, animal feed, and textile industries. The potential of partially purified xylanase from Thermomyces lanuginosus MC 134, Luminase PB 100, Luminase PB 200 (a commercial xylanase) and T. lanuginosus DSM 5826 (Sigma Aldrich) was evaluated in bleaching of bagasse pulp. The temperature and pH optima for all the enzymes were 60°C and pH 6, respectively. The temperature (50- 80°C) and pH (5-8) stability of the enzymes were also assessed. All the enzymes were relatively stable at 60°C and pH 6 for 180 min. T. lanuginosus MC 134 retained 80% of its activity at 60°C and pH 6 for 180 min and PB 200 retained 75% of its activity at 80°C for 180 min. T. lanuginosus MC 134 also exhibited good alkaline stability at pH 8. The commercial xylanases Luminase PB 100, Luminase PB 200, T. lanuginosus DSM 5826 (Sigma Aldrich) were purified to homogeneity using a gel filtration column packed with sephadex G-100 and characterized for Km and Vmax. However extracellular crude xylanases from T. lanuginosus MC 134 was purified to homogeneity using (N )2S04 precipitation and gel filtration column, packed with sephadex G-100. The purified xylanases exhibited a molecular mass of- 26 to 24 kDa, given range as determined by SDS page. The Km and Vmax values of Luminase PB 100, Luminase PB 200, T. lanuginosus MC 134, and T. lanuginosus DSM 5826, xylanases were determined by the Michaelis-Menten equation using birchwood xylan as the substrate. The Km value for Luminase PB 100, Luminase PB 200, T. lanuginosus DSM 5826 and T. lanuginosus MC 134 were, 8.1 mg/mL, 11.7 mg/mL and 14.3 mg/mL respectively. The Vmax for Luminase PB 100, Luminase PB 200, T lanuginosus DSM 5826 and T lanuginosus MC 134 were 232.6, 454.6 and 74.6 !Jl11ol/min/mg. Biobleaching conditions of the xylanases were also optimised and the release of reducing sugars and lignin derived compounds showed that an enzyme dosage of 50U/g of pulp was ideal for biobleaching at pH 6 and 60°C for 180 min. This brightness for T lanuginosus MC 134, Luminase PB 200, Luminase PB 100 was 45.5 ± 0.11%, 44.1 ± 0.007% and 42.7 ± 0.03% respectively at pH 6, compared to untreated samples. Reducing sugars and UV-absorbing lignin-derived compound values were considerably higher in xylanase-treated samples. All the enzymes analysed exhibited similar trends in the release of lignin derived compounds and reducing sugars which indicated their potential in the pulp and paper industry.Item The chitinolytic enzyme system of the compost-dwelling thermophilic fungus Thermomyces lanuginosus(2014) Zhang, Meng; Singh, Suren; Permaul, Kugen; Wang, Zheng-XiangChitin, a highly insoluble 1,4- -linked polymer of N-acetyl- -D-glucosamine, is the second-most abundant bio-polysaccharide in nature after cellulose. Most chitinolytic fungi are known to produce more than one kind of chitinase. The recent sequencing of the Thermomyces lanuginosus SSBP genome by our group has revealed four putative family 18 chitinases. In this study, three novel chitinase genes (chitl, chit2 and chit3) and the previously reported chit4 gene were cloned from Thermomyces lanuginosus SSBP and their gene structures were analysed. chit3, encoding a 36.6 kDa protein, and chit4, encoding a 44.1 kDa protein, were successfully expressed in Pichia pastoris. The recombinant Chit3 and Chit4 enzymes exhibited optimum activity at pH 4.0 and 5.0 and at 40oC and 50°C, respectively. Chit3 was stable at 40oC and retained 71% of its activity at 50°C after 60 min, while Chit4 was stable at 50°C and retained 56% of its activity at 60°C after 30 min. Both enzymes produced chitobiose as the major product using colloidal chitin, chitooligosaccharides and shrimp shell powder as substrates. Of the fungal strains tested, Chit3 displayed antifungal activity against Penicillium sp. and Aspergillus sp. This is the first report on the multi-chitinolytic system of T. lanuginosus and enzyme characterization has shown the potential of the enzymes to be used in degradation of the under-utilized bio-resource chitin.