The extent of diffusion and surface modification of a purified 23.6 kDa xylanase isolated from Thermomyces lanuginosus on bagasse pulp was evaluated. Polyclonal anti-xylanase antibodies were raised in two rabbits and in conjunction with immunogold labeling and microscopic studies enzyme diffusion and degradation studies were performed. The purity of the xylanase was confirmed by SDS-PAGE and western blots confirmed the antigen-antibody hybrid on the nitrocellulose membrane.
