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Research Publications (Applied Sciences)

Permanent URI for this collectionhttp://ir-dev.dut.ac.za/handle/10321/213

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Author: search.filters.author.Singh, RishanSubject: search.filters.subject.Apoptosis

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    Molecular immunogenetics of apoptosis : experimental dilemas
    (International Journal of Biological & Pharmaceutical Research, 2012) Singh, Rishan; Reddy, Lalini
    There have been several research articles published on the biological and biochemical nature of apoptosis. These have included studies on the molecular genetics of apoptosis. Apart from the genes that are involved in the apoptotic cascade, there are several other genes that are either activated or inhibited when cell lines are exposed to apoptotic stimuli. This article addresses the simplicity and complexity of the genetic nature of apoptosis in a variety of cell lines.
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    Apoptosis in the human laryngeal carcinoma (HEp-2) cell line by Bulbine natalensis and B. Frutescens fractions
    (IJBPR, 2012) Singh, Rishan; Reddy, Lalini
    Many plants that belong to the genus Bulbine have compounds in their roots and leaves which are considered important for traditional treatments. The stems and roots of Bulbine species are believed to contain anticancer compounds such as anthraquinones, including chrysophanol and knipholone. However, in general, people utilise plants of the Bulbine genus for the treatment of rashes, itches, wounds, burns, cracked lips and cracked skin. This study assessed the effect of aqueous and organic fractions of Bulbine natalensis and Bulbine frutescens on the human laryngeal carcinoma cell line (HEp-2) for apoptosis. The MTT assay was used to determine the cytotoxicity of the fractions administered and to select fractions for analysis of bax and caspase-3 gene expression, which are induced during programmed cell death type 1, known as apoptosis. All of the B. natalensis fractions induced expression of caspase-3, while the tested B. frutescens aqueous root fractions failed to induce expression of caspase-3. The variation in bax gene expression indicated that HEp-2 cell death was due to apoptosis and other unknown forms of cell death that may or may not activate caspase-3 gene expression.