Research Publications (Applied Sciences)
Permanent URI for this collectionhttp://ir-dev.dut.ac.za/handle/10321/213
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Item 7 Unlocking nature’s remediation arsenal : the role of polyphenol oxidases in efficient and eco-friendly industrial wastewater treatment(De Gruyter, 2024-04-08) Nagarajan, Prithiviraj; Rajathy, Leena; Patil, Sharangouda J.; Golla, Narasimha; Krishna, Suresh Babu NaiduPhenol and its derivatives have gained considerable attention recently due to their high toxicity, teratogenicity, and mutagenicity. Petroleum refinery wastewater is a significant source of phenolic compounds. However, conventional techniques used to treat these wastewaters have several drawbacks, such as incomplete or inefficient removal of phenols. In contrast, biocatalytic processes have garnered significant attention as they offer sustainable and effective removal of toxins, including phenols, from wastewater. Among various biocatalysts, polyphenol oxidases have emerged as major biocatalytic enzymes. These enzymes contain copper and catalyze the oxidation of specific phenolic substrates to quinones in the presence of molecular oxygen. Polyphenol oxidases have a wide range of applications. In the food industry, they are utilized for cocoa and tea production, enhancing coffee flavor and assessing food quality. In medicine, they find applications in treating phenylketonuria, Parkinson's disease, and leukemia. In environmental technology, they play a crucial role in removing phenolic pollutants from industrial wastewater. In the pharmaceutical industry, polyphenol oxidase-immobilized electrodes differentiate between morphine and codeine. This chapter provides comprehensive details about polyphenol oxidases' structure, biochemical properties, and applications, specifically focusing on their role in wastewater treatment.Item 12 Polyphenol oxidases : the future toward global sustainability(De Gruyter, 2024-04-08) Harun-Ur-Rashid, Mohammad; Krishna, Suresh Babu Naidu; Golla, Narasimha; Bin Imran, AbuPolyphenol oxidases (PPOs) are enzymes that catalyze the oxidation of phenolic compounds, which are abundant in many plant-based foods. PPOs are crucial in postharvest losses and food waste, especially in developing countries with underdeveloped food supply chains. There has been a growing interest in utilizing PPOs for sustainable food production and preservation, modifying phenolic compounds to develop new food products, detecting phenolic compounds in various products, and utilizing bioremediation, agriculture, biotechnology, and waste management techniques to promote global sustainability. These advances have the potential to provide effective solutions toward achieving a more sustainable future. The most promising application of PPOs for achieving global sustainability is their use as a natural preservative to prolong the shelf life of fresh produce. They can be used to produce novel food products, such as functional foods and nutraceuticals, by modifying the phenolic compounds. The approach can add value to the food industry by creating new products with health benefits and reducing waste. PPOs can be used in bioremediation processes to degrade phenolic compounds found in industrial wastewater and produce natural antioxidants from food waste, promoting circular economy principles. They can also contribute to sustainable agriculture by increasing plant resistance to pests and diseases, reducing synthetic pesticides and herbicides, and improving crop yields. Overall, PPOs have a promising role in creating a more sustainable environment. This chapter thoroughly examines the latest developments in utilizing PPOs for sustainable food production and waste management. It emphasizes the enzyme's potential in natural preservation, novel food production, bioremediation, and sustainable agriculture. Additionally, the authors explore the wide range of applications for PPOs, such as biosensors, bioremediation, agriculture, and biotechnology.Item Green synthesis of silver nanoparticles using flower extracts of Aerva lanata and their biomedical applications(Informa UK Limited, 2022-01-02) Palithya, Sashikiran; Gaddam, Susmila Aparna; Kotakadi, Venkata Subbaiah; Penchalaneni, Josthna; Golla, Narasimha; Krishna, Suresh Babu Naidu; Naidu, C.V.In the present study, green synthesis of silver nanoparticles (AgNPs) was performed using flower extracts of Aerva lanata (Al) from Amaranthaceae family. The size, shape, and elemental composition of the silver nanoparticles (AgNPs) was studied using transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), and UV-visible spectroscopy. The dynamic light scattering (DLS), of silver nanoparticles (AgNPs) exhibited mean size of 7.6 nm in the range of 5–15 nm, having a poly-dispersed indexed value of 0.419. Zeta potential contributing to the stability of silver nanoparticles was recorded as −18.7 mV. Transmission electron microscopy (TEM) and energy dispersive X-ray (EDX) analysis confirmed the presence of the synthesized AgNPs with an average size range 7 ± 3 nm. The biosynthesized silver nanoparticles displayed antibacterial activity against Gram-positive and Gram-negative bacteria. The 2,2’-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay revealed antioxidant potential of silver nanoparticles in the concentration of 100 µg/ml. The photocatalytic activity of silver nanoparticles was evaluated using decolourization of the dyes under the sunlight using UV-Vis. spectroscopy. The results suggest that biosynthesized silver nanoparticles from A. lanata (Al-AgNPs) flower extracts have broad spectrum antibacterial, antioxidant and catalytic activities may be useful in a variety of biomedical and industrial applications.Item Effect of lignocellulosic materials and chlorpyrifos pesticide on secretion of ligninolytic enzymes by the white rot fungus – Stereum ostrea(Taylor and Francis, 2022-02-15) Shanthi Kumari, B. S.; Kanderi, Dileep Kumar; Golla, Narasimha; Krishna, Suresh Babu Naidu; Sai Geetha, K.; Vyshnava, Satyanarayana Swamy; Rajasekhar Reddy, B.The present study compared the effect of chlorpyrifos on secretion of ligninolytic enzyme by the white rot fungus – Stereum ostrea grown in the presence and the absence of Tectona grandis cubes under submerged liquid conditions. Significantly higher yields of lac case and manganese peroxidase to the extent of 164.30 and 59.28 U/ml were registered in control containing only teak wood cubes than in the culture on combination of cubes and chlorpyrifos with 111.65 and 47.24 U/ml, respectively on 10th day of incubation. Reversed pattern in lignin peroxidase secretion with peak values on 6th day of incubation was noticed in the culture of S. ostrea immobilized on Tectona grandis cubes under influence of chlorpyrifos. Under free state conditions, significantly higher secretions of laccase (125.33 U/ml) and manganese peroxidase (53.60 U/ml) occurred in chlorpyrifos-amended medium than in the medium devoid of chlorpyrifos on 10th day incubation. Under moderate shaking conditions, supplementation of wheat bran alone at 3% level to the medium resulted in enhanced (3–5 folds) secretions of laccase, manganese peroxidase and lignin peroxidase by S. ostrea in medium free of wheat bran on 12th and 10th day of incubations. The presence of chlorpyrifos in addition to wheat bran in the medium further raised production of ligninolytic enzymes – laccase, MnP and LiP to the level of 311.78, 130 and 3 U/ml respectively on the 12th (higher LAC and MnP secretions) and 10th (higher LiP secretions) day of incubations. Our data in the present study suggest that secretion of ligninolytic enzymes by the culture is dependent on the nature of lignocellulosic material used in the study and secretion of higher titers of ligninolytic enzymes on cubes of Tectona grandis in the absence of chlorpyrifos rather in the presence of chlorpyrifos occurred. For executing microbial applications, it is critical to understand how lignocellulosic materials and toxic xenobiotic pesticides interact with white rot fungi and possible role of ligninolytic enzymes in the bioremediation processItem Biosynthesis and characterization methods of copper nanoparticles and their applications in the agricultural sector(Elsevier, 2022-01-15) Reddy, Bhagavanth; Mangatayaru, Girija K.; D, Madhusudan Reddy; Krishna, Suresh Babu Naidu; Golla, Narasimha; Kamel A, Abd-ElsalamThis book provides information on the design and application of ecologically safe formulations, for protecting cultivated crops against insects and pathogen-causing diseases, plant science, antimicrobial applications and agroecosystem ...