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Research Publications (Applied Sciences)

Permanent URI for this collectionhttp://ir-dev.dut.ac.za/handle/10321/213

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Author: search.filters.author.Nizam, AatikaSubject: search.filters.subject.Sclerotium rolfsii

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    Differential approach of bioremediation by sclerotium rolfsii towards textile dye
    (2023-10-05) Samuel, Anthony; Lakshmaiah, , Vasantha Veerappa; Dias, Priyanjali; Praveen, N.; Fernandes, Cannon Antony; Nizam, Aatika; Krishna, Suresh Babu Naidu
    Synthetic dyes are extensively used in various industries and are one of the major contaminants of industrial effluents. Dyes being xenobiotic, carcinogenic, and toxic there is need for their effective removal and detoxification to conserve water resources. Tremendous research has been carried out to identify potent microorganisms that facilitate bioremediation of these harmful dyes. A static batch culture has proved white rot fungi Sclerotium.rolfsii as an efficient catalyst in bioremediation of textile dyes and to compare their efficiency in decolourisation of two different azo dyes. Studies revealed the organism employ different remedial approach to cationic dye (Malachite green) and anionic dyes (Rose Bengal). Decolourisation of malachite green was a gradual with degradation and bio-transformation to colourless, non-toxic by products while Decolourisation of rose Bengal was quick process of biosorption. S.rolfsii exhibited 89% of decolourisation of malachite green dyes at higher concentration of 900mg/L while 96% for rose Bengal at 900mg/L. The mechanism of dye decolourisation was proposed using the UV Vis spectrophotometry, FTIR, XRD, HPLC and SEM. Microbial toxicity studies confirmed the dye metabolites of degraded malachite green was less toxic compared to original dye. Com- prehensively studies illustrate the sustained application of S. rolfsii as model organism for bioremediation of complex industrial effluents due to its differential bio remedial approach can potentially decolourise or remove various dyes.
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    Fungi-templated silver nanoparticle composite: synthesis, characterization, and its applications
    (MDPI AG, 2023-02-06) Joy, Francis; Devasia, Jyothis; Nizam, Aatika; Veerappa Lakshmaiah, Vasantha; Krishna, Suresh Babu Naidu; Krishna, Suresh Babu Naidu; Anthony William, Coleman
    The self-assembly of nanoparticles on living bio-templates is a promising synthetic methodology adopted for synthesizing nano/microstructures with high efficiency. Therefore, the method of bio-templating offers various advantages in controlling the geometries of nano/microstructures, thereby increasing the efficiency of the synthesized material towards various functional applications. Herein, we utilized a filamentous fungus (Sclerotium rolfsii) as a soft bio-template to generate silver nanoparticle (AgNP) microtubules adhering to the fungal hyphae. The resulting composite combines the unique properties of silver nanoparticles with the biological activity of the fungi. The 3D fungal hyphae–silver nanoparticle (FH-AgNP) composite was characterized using SEM, elemental analysis, and the X-ray diffraction technique. Additionally, to highlight the functional application of the synthesized composite, dye degradation studies of methylene blue under visible light was effectuated, and a percentage degradation of 67.86% was obtained within 60 min, which highlights the potent catalytic activity of FH-AgNPs in dye degradation. Further, the antibacterial study of the composite was carried out against the bacterium Escherichia coli, and it was found that 200 µg of the composite exhibited maximum antibacterial properties against Gram positive (Staphylococcus aureus) and Gram negative (Escherichia coli) bacteria. Overall, fungi-templated silver nanoparticle composites are a promising area of research due to their combination of biological activity and unique physical and chemical properties.