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Research Publications (Applied Sciences)

Permanent URI for this collectionhttp://ir-dev.dut.ac.za/handle/10321/213

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    Microbial production of phytases for combating environmental phosphate pollution and other diverse applications
    (Taylor and Fancis Online, 2016) Kumar, Ashwani; Chanderman, Ashira; Makolomakwa, Melvin; Perumal, Kugen; Singh, Suren
    Concerns of phosphorus pollution and its impact on environments have driven the biotechnological development of phytases. Phosphoric acid, inositol phosphate, or inositols are produced after hydrolysis of phosphate from phytate, initiated by phytase. Research over the last two decades on microbial phytases has deepened our understanding of their production, optimization, and characterization. Despite the wide availability of phytase producing microorganisms, only a few have been commercially exploited. The current high cost of phytases, inability to withstand high temperatures (>85 C), a limited pH range, and poor storage stability are a major bottleneck in the commercialization of phytases. The development of novel phytases with optimal properties for various applications is a major research challenge. In this paper, recent advances in microbial phytase production, application of tools to optimize higher enzyme production, and characterization of phytases along with potential biotechnological applications are reviewed. Additionally the development of phytase assay methods and functions of phytate and phytate degradation products are discussed.
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    Improvement of ethanol production from sugarcane molasses through enhanced nutrient supplementation using Saccharomyces cerevisiae
    (Academic Journals, 2012-03) Nofemele, Zuko; Shukla, Pratyoosh; Trussler, Arthur; Permaul, Kugen; Singh, Suren
    Saccharomyces cerevisiae as a yeast cream was utilized for alcoholic fermentation using sugar cane molasses. In the present study, fermentation was optimized for urea and yeast hydrolysate (YH) dosage and the combined effect was evaluated. Total sugars as inverts (TSAI) composition of molasses were -1 determined by HPLC as 39% (m/v). Urea concentrations of 4, 2 and 3 gl showed optimal ethanol -1 production at 30, 35 and 40°C respectively. A YH concentration of 0.5 gl resulted in an ethanol yield of 8.7% (m/v) with a fermentation efficiency of 85.12%. Under optimized conditions (35°C) significant improvements were noticed with ethanol yield of 7.8% (m/v) and efficiency of 76.3%.