Faculty of Health Sciences
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Item The effect of camphor, naphthalene (moth balls), and electromagnetic radiation on 4cH and 200cH homoeopathic potencies of gibberellic acid and their subsequent ability to promote germination of barley seed (Hordeum Vulgare), as measured by a germination index(2002) Stubbs, Claire; Steele, RichardThis study investigated the effect of camphor, naphthalene (mothballs), cellular phones and televisions on 4cH and 200cH homoeopathic dilutions of gibberellic acid (GA3).The ability of treated dilutions to promote germination of barley seed, (Hordeum vulgare cv. Stirling, ex Sensako, Western Cape, South Africa, 2000 harvest) was measured by a germination index.Item The efficacy of different methods of preparation of ultra high dilutions of gibberellic acid on the synthesis of alpha-amylase in de- embryonated endosperm halves of barley seed(2002) Balding, Tamara Jane; Steele, RichardThe mode of action of homoeopathic remedies is not well understood and is questioned by the scientific community due to the high degree of dilution of such remedies. The method by which remedies are produced is thought to instill the efficacy into these remedies. This research examines whether effective homoeopathic remedies may be prepared by electromagnetic induction, by serial dilution alone, or by conventional Hahnemannian production, which includes succussion. These methods were investigated in terms of the ability of dilutions of gibberellic acid, prepared by the above methods, to induce production of a-amylase by de-embryonated endosperm halves of barley seed.Item The effect of ultra high dilutions of gibberellic acid on the synthesis of a-amylase in de-embryonated halves of barley seed (Hordeum vulgare stirling)(1999) Steele, Richard; Cairns, A. L. P.This study investigated the effect of ultra high dilutions of gibberellic acid (GA3) on the synthesis of the enzyme a-amylase in de-embryonated endosperm halves of barley seed (Hordeum vulgare cv. Stirling, ex Caledon, Western Cape, South Africa, 1998 harvest), in order to determine whether such dilutions are capable of producing a biological effect or not. Homoeopathic theory and practice proposes that such dilutions are capable of such effects (Gaier 1991 :445-447).Item The effect of light and heat on 4CH and 200CH potencies of gibberellic acid as a promoter of germination in barley seed (Hordeum vulgare), measured by a germination index(2002) Pieterse, Hayley; Steele, RichardThe purpose of this investigation was to evaluate the effects of light and heat on homoeopathic dilutions of gibberellic acid (cHGA3) as measured on Hordeum vulgare (barley) seed germination.Item The effect of light and heat on 4CH and 200CH potencies of gibberellic acid as a promoter of germination in barley seed (Hordeum vulgare), measured by a germination index(1999) Kruger, Theodorus Hendrik; Naicker, V.A search of the literature failed to reveal previous studies performed to determine the effect of spinal manipulation in chronic low back pain sufferers, in terms of clinical and immune cellular responses. Previous studies have dealt with either clinical or immune cellular responses but not both in the same studyItem The effect of homoeopathic potencies of abscisic acid on the production of a-amylase in barley seeds (Hordeum vulgare) in the presence of gibberellic acid(2001) Couchman, Ingrid Marcelline Stephanie; Cairns, A. L. P.This study investigated the effect of different homoeopathic potencies of abscisic acid (ABA) in the presence of gibberellic acid (GA3) on the production of a.-amylase in the de-embryonated endosperm half-seeds of barley (Hordeum vulgare) in order to investigate whether these potencies were able to produce a biological effect. The aleurone layer of the barley (Hordeum vulgare) grain secretes hydrolases that mobilise endosperm reserves during germination. The synthesis and secretion of these hydrolases (principally a.-amylase) is under hormonal regulation. Gibberellic acid (GA) stimulates the synthesis and secretion of a.-amylase and abscisic acid (ABA) reverses this effect. It is for this reason that barley aleurone has been used extensively as a model system for the study of signal transduction in response to GA and ABA (Ritchie, McCubbin, Ambrose, Kao and Gilroy, 1999). For this research five different homoeopathic potencies were used namely - the 4CH, 9CH, 15CH, 30CH and the 200CH. Two methods were used in this experiment namely - spectrophotometry and radial diffusion. For each method three replications were used. Each replication consisted of: five groups of 20 half-seeds treated with the various potencies (the 4CH, 9CH, 15CH, 30CH and the 200CH) of ABA, with a dilution at GA3 at 1x 10.g;five groups of 20 half-seeds treated with the of GA3 at 1X 10.9 (the control) and five groups of 20 half-seeds treated with only the incubation buffer (calcium nitrate). An a.-amylase curve was constructed for both methods using pure a.-amylase at 1350 enzyme units /mgItem The effect of homoeopathically prepared dilutions of gibberellic acid on the germination of barley seed (Hordeum vulgare L.) as measured by a germination index(2001) Lok, Karen Him; Hamman, Brigitte; Hopkins, Crofton RussellThe potentisation process during which homoeopathic preparations are produced, raises the concern that these remedies have a placebo effect, since they contain no active molecule of the substance used to prepare them (in ultra high dilutions) by the time they are administered to a patient. Plant models therefore offer a more direct method of examining the efficacy of homoeopathically prepared solutions. This study investigated the effects of homoeopathic preparations of gibberellic acid on the germination of barley seeds (Hordeum vulgare L.) as measured by a germination index. The effects of GA3 at potencies of 4cH, lScH, 30cH and 200cH on germination rate and seedling development were tested on barley seeds of high-, medium- and low- vigour. The index used comprised the rate at which seeds germinated, which was the time it took for 50 percent of those seeds that did germinate, to germinate (i.e. Tso); seedling development experiments as assessed by taking final germination counts 7 days after start of imbibition, shoot and root lengths, and seedling dry mass. Biological activity of homoeopathically prepared GA3 at lScH was evident in medium vigour seeds, since this treatment resulted in significantly long root development. Highvigour barley seeds imbibed in GA3 (0.5 g L\xB7l ) were found to germinate the fastest of all treatment groups, and high-vigour seeds imbibed in homoeopathically prepared GA3 at 4cH, 30cH and 200cH, germinated faster than those seeds not receiving GA3 in any form (the control). Amongst high-vigour seeds, prior imbibition with homoeopathicallyItem A comparison between the efficacy of radionically prepared gibberellic acid and homoeopathically prepared gibberellic acid (GHP) on the germination rate and seedling development of barley seeds(2016) Kleingeld, Gerhard; Naude, David F.Aim The aim of this controlled, experimental study was to compare the biological activity of various homoeopathic potencies of gibberellic acid manufactured radionically (AMS transfer device) and conventionally (GHP) in terms of their respective influence on germination rate and seedling development of barley seeds; all the respective results being contrasted against those produced by the distilled water control. Methodology The research was completed by employing quantitative research techniques and followed true experimental design. Homoeopathically (Hahnemannian) prepared gibberellic acid followed the manufacturing guidelines of method 5a involving liquid preparations, as specified in the German Homoeopathic pharmacopoeia (GHP) (Benyunes 2005). A second radionic ‘equivalent’ version of each of the Hahnemannian potencies was manufactured using the ‘AMS wave transfer’ device. Four sources of data were collected namely, germination count and rate, seedling development (root length), seedling dry mass, and number of seeds with measurable roots. All the data was collected and documented on a data collection sheet using Microsoft Excel. All the data was statistically analysed and subjected to analysis of variance (ANOVA) using GenStat Version 14 (VSN International, UK) at the 5% level of significance. The statistical data was used to produce a comparison between the different remedies and distilled water. Results All of the remedy treatment groups (Radionic 200c, Hahnemannian 200cH, Radionic 4c and Hahnemannian 4cH) displayed suppressive effects (to certain extents respectively ) on seed growth and development in comparison to the control group (distilled water). The control group displayed greater seedling development in comparison to all remedy treatment groups which was most evident in the average root lengths and high vigour seed lot root lengths having longer roots than all remedy treatment groups. The control group also displayed a higher number of seeds with measurable roots compared to all the remedy treatment groups in both total number of seeds and in the seeds accounted for in the high vigour lots. This suggests that all Homoeopathic remedies irrespective of potency or manufacture method (Radionic or Hahnemmanian) had similar suppressive effects on root growth and seedling development and this suppressive effect was in turn not evident in the control group. Conclusion The experiment results suggest that radionically manufactured homoeopathic remedies (AMS wave transfer device) have similar biological effects (suppressive effects) to the equivalent Hahnemannian manufactured homoeopathic remedies, although further research in this field is necessary to confirm these findings the results from this study are supportive of the use of radionically prepared remedies in homoeopathic practice.