Faculty of Applied Sciences
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Item The isolation and characterization of phytoalexin and constitutive agents from plants for mycotoxin control(2000) Mohanlall, Viresh; Odhav, BhartiPlant medicine is an important area of commercial activity in South Africa. This is a rapidly expanding market, thus we are evaluating natural and stressinduced compounds (phytoalexins) from plants as agents that may be able to control mycotoxins. Natural compounds from Bridelia micrantha, Warburgia salutaris, Lippia javanica and Scenecio serratuloides and stress-induced compounds (phytoalexins) from Citrus sinensis cv Valencia were screened for antitunqal and antimycotoxic activity by bioautography against a test organism (Cladosporium cladosporoides) and mycotoxin producing fungi (Fusarium moniliforme and Aspergillus flavus).Item The effect of thermal processing on fumonisin B1 (FB1) levels in maize-based foods(Academic Journals, 2013-03-15) Odhav, Bharti; Mohanlall, Viresh; Mohanlall, RakeshFumonisin B1 (FB1) is a mycotoxin from Fusarium verticillioides that is frequently associated with maize. Fumonisins have been implicated as the causal agents of a variety of animal diseases and are epidemiologically linked to the high incidence of human esophageal cancer in some regions of the world. Thermal treatments are used in many processes involving grain and its derivatives, but little is known about the effects of common processing methods on the fumonisin content of food. The objectives of this study were to determine the thermostability of this toxin in contaminated maize, at different time/temperature combinations, as well as to determine the effect of baking and frying on the stability of FB1 spiked into maize-based foods. The identity of FB1 in extracts before and after heat treatments was confirmed by high-performance liquid chromatography. For each thermal process, the fumonisin content was inversely proportional to the processing temperatures. An initial FB1 concentration of 217 mg/g in the control, was reduced to 184 mg/g when treated at 100°C for 2 h. Oven temperature of 220°C for 30 min showed extensive reduction of FB1 to a concentration of 1.1 mg/g as compared to 94 mg/g in the control. Baking maize muffins spiked with 1.25 μg/g (dry weight) FB1 at 200°C for 20 min resulted in an average FB1 loss of 70%. Frying of maize chips spiked with 5 μg/g (dry weight) FB1 at 190 to 210°C for 5 to 10 min resulted in an average FB1 loss of 67%. The results of this study indicate that boiling temperatures are ineffective in producing any significant reduction in FB1 levels. Thus, there might be correlation between the under processing of fumonisin-contaminated foods and the high incidence of esophageal cancer in certain regions of South Africa.