Faculty of Applied Sciences
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Item Evaluation of chitosan–coated magnetic nanoparticle-immobilized thermostable hemicellulases for enhanced saccharification and production of bioethanol(2022-09) Mdlaka, Sibongile Patience; Singh, Suren; Puri, Adarsh KumarEnhancing the efficiency of saccharification of pentose and hexose sugars present in lignocellulosic biomass is a major bottleneck for industrial bioethanol production. This problem can be addressed by a concerted effort combining nanotechnology, enzymology and fermentation technology. Functionalized chitosan-coated magnetic nanoparticles (CCMNPs) were prepared and used for co-immobilization of purified xylan hydrolysing xylanase and xylosidase from the thermophilic fungus Thermomyces lanuginosus SSBP for the release of xylose. Stability studies revealed that immobilized enzymes were more stable than free enzymes over a wide range of pH (4.0 – 7.0) and temperature (40 – 90 °C) for xylanase and 30 – 80 °C for xylosidase. The optimum activity of the co-immobilized enzymes shifted slightly as compared to the free enzymes, with coimmobilized xylanase and xylosidase showing optimum activity at pH 6.5 and 6.0, respectively. The study showed sustained production of xylose as the major fermentable sugar under repeated batch and fed-batch saccharification of lignocellulosic biomass. Statistical optimization of saccharification of 1% xylan using response surface methodology indicated the enhanced release of xylose at 50 °C, pH 7.0 and enzyme dose of 60 U/mL xylanase and 30 U/mL xylosidase. Finally, liberated xylose was fermented with Scheffersomyces stipitis to yield bioethanol.Item Investigations of the bioprocess parameters for the production of hemicellulases by Thermomyces lanuginosus strains(2012-08-17) Pillai, Santhosh Kumar Kuttan; Singh, Suren; Permaul, KugenThe aim of this study was to evaluate T. lanuginosus for the production of hemicellulases, its yield enhancement using mutagenesis and application of a selected xylanase on bagasse pupl to assess the improvement of pulp properties. The objectives were: To determine the localization of hemicellulases in T. lanuginosus strains, To develop high yielding strains of T. lanuginosus through mutagenensis, To investigate the synthesis of xylanase by T. lanuginosus MC134, To optimize the medium components and cultural conitions of T. lanuginosus MC134 strain, To study the influence of agitation and aeration on the production of xylanase by T. lanuginosus MC134 in a fermenter, To evaluate the bleach boosting abilities of T. lanuginosus xylanase on bagasse pulp, To evaluate simultaneous xylanase production and biobleaching potential of T. lanuginosus.