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    Bio-decolorization and degradation of reactive blue 222 by a novel isolate Kucoria marina CU2005
    (Association of Biotechnology and Pharmacy, 2023-01-30) Veerappa Lakshmaiah, Vasantha; Krishna, Suresh Babu Naidu; S More, Sunil; K Jayanna, Shobha
    In this study, a novel bacterial strain, Kucoria marina CU2005, was isolated and identified using 16S rRNA gene sequencing from an industrial wastewater sludge sample capable of degrading Reactive Blue 222 (RB222) dye. Batch mode bio stimulation studies were performed with minimal salt media to optimize key physiological parameters for effective decolorization of RB222. When cultured at 35 °C and pH 7 under static conditions, this bacterium decolorized 82 percent of the dye after 24 hours. Decolorization was monitored using UV-vis spectrophotometry. Isolate’s ability to decolorize the complex dye was attributed to its degradation potential rather than a passive surface adsorption. FTIR, HPLC, GC-MS studies were used to confirm microbial dye metabolism. The results indicated breakdown of dye upon decolorization as some peaks were shifted and generation of aromatic amine for monosubstituted benzene ring as intermediates of dye degradation in decolorized solutions. This study has shown the potential of Kucoria marina CU2005 to decolorize RB222 dye at a better pace and efficiency than previously reported bacterial strains. Thus, we propose that our isolated strain can be utilized as a potential dye decolorizer in environmental biotechnology as effluent treatment for decolorization of RB 222.
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    Innovative pedagogy: implications of genetic decompositions for problem solving in management courses
    (Informatics Publishing Limited, 2014-12) Brijlall, Deonarain
    Mathematics is a subject on which many business and management courses rely. In this paper the researcher reports on the processes involved in the design of itemised genetic decompositions for certain mathematics tasks. The manner by which this is done may be extended to teaching and learning in management courses in higher education. The researcher used two tasks, one in a high school context from a previously published study and another from a qualitative case study which explored the development of mental constructions of a group of fourth-year pre-service teachers during definition making of certain mathematical concepts in real analysis, at a South African higher education institution. Questionnaires based on two items involving the concept of infinity were administered to those participating pre-service teachers. The pre-service teachers were allowed to respond to these items without lecturer involvement so that a learner-centred learning environment was created. Their mental constructions of these concepts were analysed by using an APOS (Action-Process-Object-Schema) framework. Forty six pre-service teachers, specialising in the teaching of mathematics in the FET school curriculum, participated in the project. It was found that the APOS designed questionnaire generated thick data that led to a modification of the initial genetic decomposition formulated. This modified genetic decomposition has didactical implication for business and management courses taught in higher education. This is illustrated in the recommendations section of this paper.