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    Cultivation of Chlorella pyrenoidosa in outdoor open raceway pond using domestic wastewater as medium in arid desert region
    (Elsevier, 2016) Dahmani, Siham; Zerrouki, Djamal; Ramanna, Luveshan; Rawat, Ismail; Bux, Faizal
    Chlorella pyrenoidosa was cultivated in secondary wastewater effluent to assess its nutrient removal capa-bilities. Wastewaters were obtained from a wastewater treatment plant located in Ouargla, Algeria. The experiments were conducted in winter under natural sunlight in an outdoor open raceway pond situated in the desert area. The highest biomass of the microalgae was found to be 1.71 ± 0.04 g/L. Temperatures ranged between 18 and 31 C. The average annual insolation was no less than 3500 h with an annual solar irradiance of more than 2000 kWh/m2. Analyses of different parameters including COD, NH4-N and TP were conducted throughout the cultivation period. Their average removal efficiencies were 78%, 95%and 81% respectively. The results demonstrated the potential of nutrient removal by microalgae grown on secondary wastewater in arid areas.
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    The optimization of biomass and lipid yields of Chlorella sorokiniana when using wastewater supplemented with different nitrogen sources.
    (Pubmed, 2014-09) Ramanna, Luveshan; Guldhe, Abhishek; Rawat, Ismail; Bux, Faizal
    The potential of nitrogen sources supplementing domestic wastewater for the cultivation of microalgae was assessed. Urea, potassium nitrate, sodium nitrate and ammonium nitrate were evaluated for their effect on cultivation and lipid production of Chlorella sorokiniana. Urea showed the highest biomass yield of 0.220 g L−1 and was selected for further experimentation. Urea concentrations (0–10 g L−1) were assessed for their effect on growth and microalgal physiology using pulse amplitude modulated fluorometry. A concentration of 1.5 g L−1 urea produced 0.218 g L−1 biomass and 61.52% lipid by relative fluorescence. Physiological stress was evident by the decrease in relative Electron Transport Rate from 10.45 to 6.77 and quantum efficiency of photosystem II charge separation from 0.665 to 0.131. Gas chromatography analysis revealed that C16:0, C18:0, C18:1, C18:2 and C18:3 were the major fatty acids produced by C. sorokiniana. Urea proved to be an effective nitrogen supplement for cultivation of C. sorokiniana in wastewater.