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Item Cultivation of Chlorella pyrenoidosa in outdoor open raceway pond using domestic wastewater as medium in arid desert region(Elsevier, 2016) Dahmani, Siham; Zerrouki, Djamal; Ramanna, Luveshan; Rawat, Ismail; Bux, FaizalChlorella pyrenoidosa was cultivated in secondary wastewater effluent to assess its nutrient removal capa-bilities. Wastewaters were obtained from a wastewater treatment plant located in Ouargla, Algeria. The experiments were conducted in winter under natural sunlight in an outdoor open raceway pond situated in the desert area. The highest biomass of the microalgae was found to be 1.71 ± 0.04 g/L. Temperatures ranged between 18 and 31 C. The average annual insolation was no less than 3500 h with an annual solar irradiance of more than 2000 kWh/m2. Analyses of different parameters including COD, NH4-N and TP were conducted throughout the cultivation period. Their average removal efficiencies were 78%, 95%and 81% respectively. The results demonstrated the potential of nutrient removal by microalgae grown on secondary wastewater in arid areas.Item Contribution of wastewater irrigation to Soil Transmitted Helminths infection among vegetable farmers in Kumasi, Ghana(National Centre for Biotechnology Information, 2016-12-06) Amoah, Isaac Dennis; Abubakari, Amina; Stenstrom, Thor Axel; Abaidoo, Robert Clement; Seidu, RazakWastewater irrigation is associated with several benefits but can also lead to significant health risks. The health risk for contracting infections from Soil Transmitted Helminths (STHs) among farmers has mainly been assessed indirectly through measured quantities in the wastewater or on the crops alone and only on a limited scale through epidemiological assessments. In this study we broadened the concept of infection risks in the exposure assessments by measurements of the concentration of STHs both in wastewater used for irrigation and the soil, as well as the actual load of STHs ova in the stool of farmers and their family members (165 and 127 in the wet and dry seasons respectively) and a control group of non-farmers (100 and 52 in the wet and dry seasons, respectively). Odds ratios were cal-culated for exposure and non-exposure to wastewater irrigation. The results obtained indi-cate positive correlation between STH concentrations in irrigation water/soil and STHs ova as measured in the stool of the exposed farmer population. The correlations are based on reinfection during a 3 months period after prior confirmed deworming. Farmers and family members exposed to irrigation water were three times more likely as compared to the con-trol group of non-farmers to be infected with Ascaris (OR = 3.9, 95% CI, 1.15–13.86) and hookworm (OR = 3.07, 95% CI, 0.87–10.82). This study therefore contributes to the evi-dence-based conclusion that wastewater irrigation contributes to a higher incidence of STHs infection for farmers exposed annually, with higher odds of infection in the wet season.Item The optimization of biomass and lipid yields of Chlorella sorokiniana when using wastewater supplemented with different nitrogen sources.(Pubmed, 2014-09) Ramanna, Luveshan; Guldhe, Abhishek; Rawat, Ismail; Bux, FaizalThe potential of nitrogen sources supplementing domestic wastewater for the cultivation of microalgae was assessed. Urea, potassium nitrate, sodium nitrate and ammonium nitrate were evaluated for their effect on cultivation and lipid production of Chlorella sorokiniana. Urea showed the highest biomass yield of 0.220 g L−1 and was selected for further experimentation. Urea concentrations (0–10 g L−1) were assessed for their effect on growth and microalgal physiology using pulse amplitude modulated fluorometry. A concentration of 1.5 g L−1 urea produced 0.218 g L−1 biomass and 61.52% lipid by relative fluorescence. Physiological stress was evident by the decrease in relative Electron Transport Rate from 10.45 to 6.77 and quantum efficiency of photosystem II charge separation from 0.665 to 0.131. Gas chromatography analysis revealed that C16:0, C18:0, C18:1, C18:2 and C18:3 were the major fatty acids produced by C. sorokiniana. Urea proved to be an effective nitrogen supplement for cultivation of C. sorokiniana in wastewater.